Prevalence of Angiostrongylus cantonensis in definitive and intermediate hosts collected from agricultural areas in Ampayon, Butuan City, Southern Philippines.

Angiostrongyliasis is a parasitic disease and a leading cause of human eosinophilic meningoencephalitis caused by rat lungworm Angiostrongylus cantonensis. This parasite infects a wide range of animal hosts, including snails and rats, which plays a significant role in zoonotic transmission. The study was conducted to determine the prevalence of A. cantonensis infection in freshwater snails and definitive rat hosts in the agricultural area in Ampayon, Butuan City, Philippines. A total of 54 rat samples and 719 snail individuals were collected in June and July 2020. An overall 2.36% prevalence rate of A. cantonensis snail infection was recorded, consisting of Pomacea canaliculata and Melanoides tuberculata, with a prevalence rate of 4.05% and 1.38%, respectively. Results revealed an overall prevalence of 38.9% in rat infection. Rattus tanezumi (48.48%) showed a higher infection than Rattus norvegicus (23.80%). Higher infection rates were found in rice field environments than residential houses, with 44.12% and 30% prevalence rates, respectively. Moreover, male rats showed higher infection rates (50%) than female rats (26.92%). Among age classes, adult rats had significantly higher infection rates (48.57%) than juvenile rats (21.05%). Correlation analysis showed a significant positive correlation between A. cantonensis infection intensity to the body length (r = 0.603; p = 0.001) and body weight (r = 0.715; p = 0.000) of rats. The study exemplifies the critical role of intermediate and definitive hosts for angiostrongyliasis. Infected freshwater snails and rats in rice fields make these agricultural areas a venue for A. cantonensis emergence. Integrated actions, health education campaigns, surveillance, hygiene, and good farming practices will help prevent the potential risk of the transmission of angiostrongyliasis in the area.

The prevailing infection of Schistosoma japonicum and other zoonotic parasites in bubaline reservoir hosts in the ricefield of lake ecosystem: the case of Lake Mainit, Philippines.

Bovines are important reservoir hosts of schistosomiasis, placing humans and animals in rice fields areas at risk of infection. This study reported the prevailing infection of zoonotic parasites from bovine feces in the rice fields adjacent to Lake Mainit, Philippines. Formalin Ethyl Acetate Sedimentation was performed on 124 bovine fecal samples from rice fields and documented eggs and cysts from seven parasites: Schistosoma japonicum, Fasciola gigantica, Ascaris sp., Strongyloides sp., Balantidium coli, coccidian oocyst and a hookworm species. Among these parasites, F. gigantica harboured the highest infection with a 100% prevalence rate, followed by hookworms (51.61%), B. coli (30.64%) and S. japonicum (12.09%), respectively. The intensity of infection of S. japonicum eggs per gram (MPEG = 4.19) among bovines is categorized as 'light.' Bovine contamination index (BCI) calculations revealed that, on average, infected bovines in rice fields excrete 104 750 S. japonicum eggs daily. However, across all ricefield stations, bovines were heavily infected with fascioliasis with BCI at 162 700 F. gigantica eggs per day. The study reports that apart from the persistent cases of schistosomiasis in the area, bovines in these rice fields are also heavily infected with fascioliasis. The study confirms the critical role of bovines as a reservoir host for continued infection of schistosomiasis, fascioliasis and other diseases in the rice fields of Lake Mainit. Immediate intervention to manage the spread of these diseases in bovines is recommended.

Prevalence of Schistosoma japonicum in bovines and Oncomelania hupensis quadrasi from ricefields surrounding Lake Mainit, Philippines.

Schistosomiasis is a parasitic disease caused by blood flukes of the genus Schistosoma and involves bovines and snails as a reservoir and intermediate hosts, respectively. This disease is endemic in Lake Mainit, Philippines, as early as 1947. The study was conducted to determine the prevalence of Schistosoma infection in ricefields adjacent to Lake Mainit by collecting Oncomelania hupensis quadrasi snails and bovine fecal samples which are then subjected to laboratory examination and analysis. A total of 345 O. quadrasi individuals and 32 bovine fecal samples were collected from six barangay rice field stations adjacent to Lake Mainit from February and July 2020. Among the six barangays, Alipao had the highest prevalence of Schistosoma in O. quadrasi (39.71%), followed by San Isidro (28.3%), Matin-ao (18%), Poblacion (15%), and Magpayang (2.5%). No Oncomelania snails were collected in the ricefields of Cuyago. Schistosoma japonicum had an overall prevalence of 14.71% in all the bovines sampled, where Cuyago had the highest prevalence (50.0%) followed by Alipao (33.33%). The intensity of infection of Schistosoma eggs per gram (MPEG = 0.70) among bovines is categorized as light. Bovine contamination index calculations revealed that, on average, infected bovines in Lake Mainit excrete ~ 55,000 S. japonicum eggs per day. The study confirms the important role of bovines as a reservoir host for schistosomiasis. The presence of both infected bovines and O. quadrasi in ricefields makes this agricultural venue an important nidus of S. japonicum emergence in Lake Mainit. An integrated approach of mass drug administration (MDA) of praziquantel, implementation of a bovine vaccine-directed control program, and providing farmers with mechanized farm equipment are highly recommended to minimize the risk of exposure and infection to schistosomiasis in rice fields adjacent to the lake.

Angiostrongylus cantonensis infection of non-native rats in Mount Makiling Forest Reserve, the Philippines.

A number of studies had been conducted on rat species diversity in the Philippines, however, there is a dearth of information on the extent of Angiostrongylus spp. infection in various ecological niches. Collection of non-native rat samples occurred in various sampling sites categorized as residential, agricultural and agro-forest in Philippine Mount Makiling Forest Reserve (MMFR) and its adjacent areas . Three species of non-native rats were captured including Rattus tanezumi, Rattus norvegicus and Rattus exulans. Of the total 90 non-native rats collected, 24.4% were found infected with Angiostrongylus cantonensis. Statistical analysis showed significant difference among rat species with highest prevalence observed in R. exulans (42.9%) followed by R. tanezumi (29.8%) and R. norvegicus (7.7%) (P = 0.047). Angiostrongylus cantonensis infection was significantly higher in adults (29.4%) than in juvenile rats (9.1%) (P = 0.050). However, results showed no significant difference in A. cantonensis infection between male (43.3%) and female (18.3%) rats and between wet (31.6%) and dry seasons (19.2%) (P > 0.05). Moreover, this study also revealed that rats from agricultural and agro-forest areas showed significantly higher prevalence than residential areas. With urbanization and the everchanging landscape of MMFR watersheds and buffer zones, zoonotic transmission can pose health threats to the local people.